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ioLight are pleased to announce the availability of a new version of the portable microscope to complement the original version. The original pocket microscope has a field of view (width of the area viewed by the microscope) of 1 mm, and resolution of 1 micron. This delivers superb quality images to an iPhone or iPad but still folds flat to fit in your jacket pocket, and will remain available for those who need the best resolution.
For some applications including animal and fish parasites, cell culture, electronic component inspection, the objects being viewed are larger than 1 mm, so a larger field of view is required.
To address this need, ioLight have developed a version of the portable microscope which doubles the field of view of to 2 mm. This change also has the advantage of extending the distance from the lens to the object (working distance) but reducing the resolution to approximately 2 microns.
The photo below shows 2 images of a pond water larvae – the left-hand image is taken with the original ioLight microscope with 1 mm field of view and 1 micron resolution, and the right-hand image shows the same larvae imaged with the new 2 mm field of view, 2 micron resolution version. The right-hand image clearly shows how more of the larvae is visible, thus making it easier to identify the larger parasites. The images in this post have been compressed for easy viewing on-line.
This new microscope looks the same as the original product, but has ‘F.O.V. 2mm’ engraved on the end of the mast to distinguish it from the original version. Other features, such as the 2 illuminators, glass sample stage, rechargeable Li-ion battery and operation with iPhone or iPad remain the same.
If you would like to buy a wide field of view version of the ioLight microscope, please contact us. You can get a wide field of view version as a reward for investing in our Crowdfunding campaign – www.crowdcube.com/ioLight
The science – Why does increasing the field of view decrease the resolution?
There is some fundamental science behind this. The ioLight pocket microscope captures 5 mega pixel (MP) images which are 2592 x 1944 pixels. To achieve 1 micron resolution each pixel must represent less than 0.5 microns on the object – this requirement is set by Nyquist’s theorem. With an image from an ioLight microscope 2592 pixels wide and a field of view of 1 mm, each pixel equates to approximately 0.4 microns.
In the new version the image is still 5MP and 2592 pixels wide, so with the 2 mm field of view this means each pixel represents approx. 0.8 microns, and so Nyquist’s theorem tell us that the resolution is limited to a little better than 2 microns.
Using Nyquist’s theorem applied to the camera chip like this only tells us what the minimum resolution is – for this resolution to be achieved in practice, the performance of the lens and illuminator has to be such that it also deliver an image with the required resolution. ioLight have carefully matched the lens and illuminator to the camera chip and resolution, such that the minimum resolution predicted by Nyquist’s theorem is achieved for most samples.